Book of abstracts
Posters

Artificial polyenzymes (ArPoly) combining proteins and polymers

Marked in green is the halogen that has been introduced by PrnC.

(+)-catechin is composed of three rings designated by letters A, B and C. Ring B is the one with the catechol moiety.

The Terpene mini-path, a very short enzymatic cascade to access the universal precursors of all terpenes.

The heterogeneous biocatalyst packed into a plug-flow column can be analyzed in operando through fluorescence microscopy to determine the enzyme kinetics

Structure and function of a CAR R-domain

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Biocatalytic synthesis of 3-substituted-2-hydroxy-4-butyrolactones 5 by tandem aldolase-ketoreductase biocatalytic reactions starting from formaldehyde 1 and 2-oxoacids 2.

PolyCYPs used to quickly generate metabolites and oxidised derivatives with enhanced properties that could widen IP coverage.

PolyCYPs providing handles for late-stage hydroxylation-fluorination strategy

Part 1. Discovery and application of SOI-catalyzed stereospecific epoxide isomerization via 1,2-methyl shift Part 2. Development of enantioselective cascades involving SOI-catalyzed epoxide isomerization and dynamic kinetic resolution

(A) Classical structure of a surfactant (1), surfactant with spacer (2), with polar part in green, and lipophilic part in grey. (B) Synthesis pathway for lipase-catalyzed synthesis of lauryl(3-hydroxypropyl) succinate.

(A) Kinetics study of formation and conversion of mono-laurylsuccinate and di-laurylsuccinate in mmol/L to lauryl(3-hydroxypropyl) in AUC. (B) Evolution of yields obtained for the synthesis of mono-laurylsuccinate and di-laurylsuccinate according to varia

Description of the cascade starting from xylose

The α, β-desaturation of cyclohexanone to 2-cyclohexen-1-one catalysed by cyclohexanone dehydrogenase (CDH) in the presence of flavin adenine dinucleotide (FAD), which is reduced to FADH2.

Schema of the one-pot chemo-enzymatic synthesis of GCA under solvent-free conditions, catalyzed through two consecutive reactions carried out by immobilized CALB onto 1-alkyl-3-methylimidazolium-based SILLPs, as a dual catalyst

Starting from P450BM3 which converts aliphatic 1-alkenes into the corresponding allylic alcohol an aldehyde selective anti-Markovnikov oxygenase (aMOx-A) was obtained in 14 rounds of directed evolution.

A, previous work on artificial [Mn]-hydrogenase with natural [Fe]-hydrogenase as the host protein; B, this work: an enantioselective [Mn]-hydrogenase for asymmetric hydrogenation

This artificial metalloenzyme displayed excellent functional group tolerance and broad substrate scope, catalyzing asymmetric transfer hydrogenation of ketones with high yield and enantioselectivity

Prenylation of aromatic compounds by RePT in the presence of dimethylallyl pyrophosphate (DMAPP) as a prenyl donor. The three-dimensional structure of RePT is displayed with the strictly conserved four tyrosine residues among DMATSs (represented as green

Improper color choices misrepresent the underlying data.

A) Superimposition of GtHNL (PDB code: 4BIF) in blue with Mn(II) in grey coordinated by four histidines and glutamine and Tm1459 (PDB code: 1VJ2) in green with Mn(II) in lime coordinated by four histidines. B) Cyanohydrin synthesis C) Oxidative cleavage.

Concentrations and ic values of gained stereoisomers. ic= isomeric content (target isomer/sum of all isomers [%]). Starting concentration of substrate: 200 mM

Optimized parameters of the carboligation step. LWC: lyophilized whole cells. CPME: cyclopentyl methyl ether. TEA: triethanolamine

Proposed pathway for the synthesis of Tulipalin A

Triheterofunctional support to coimmobilize several enzymes through three different immobilization chemistries

4CL: 4-coumarate CoA ligase; ACT: Acyl transferase

Design and crystal structure of a boronic-acid based artificial enzyme with genetically encoded boronic-acid residue catalysing oxime formation of alpha-hydroxy ketones and its subsequent directed evolution to optimise the protein environment surrounding

Aldolisation reaction catalysed by aldolase

FSA : Decameric structure

The biocatalytic route from 2-MGN to diisobuyl-2-methylglutarate.

The first step of the one-pot cascade, e.g. the conversion of 4-n-propyl guaiacol to isoeugenol is catalyzed by 4-n-propyl guaiacol oxidase expressing cells, followed by the transformation of isoeugenol to vanillin by isoeugenol oxygenase expressing cells

Experimental conditions (from bottom to top) for conversions of : pure 4-n-propyl guaiacol; lignin oil in the presence of DMSO; lignin oil in the absence of DMSO, 1g of lignin oil, and lignin oil in presence of cells in growth media.

Depolymerization steps of PET into its monomers terephthalic acid (TPA) and ethylene glycol (EG) by PETase. For simplicity, only the intermediate MHET is shown.

S238A activity in serum. For each reaction 1.5 ug mL-1 of enzyme was added to 1 mL of human serum with a substrate concentration of 2 mg mL-1. The samples were incubated at 37C. All reactions were stopped by heat inactivation (at 95C). Samples were take

HMF = 5-Hydroxymethylfurfural, DFF = 2,5-Diformylfuran, HMFCA = 5-Hydroxymethyl-2-furancarboxylic acid, FFCA = 5-Formyl-2-furancarboxylic acid, FDCA = 2,5-Furandicarboxylic acid

A redox enzyme with an N-terminal tripeptide catalyst performing the oxidation of n-hexanol to n-hexanal and a subsequent conjugated addition to a -nitroaldehyde

Cascade processes involving enzyme and organocatalysis to access chiral compounds

Selective transesterification of DHLc, a sesquiterpene lactone from chicory, using Novozyme 435. 100% conversion was obtained within 24h

Conversion (%) after 48h at 37°C, 35rpm in 1mL MBTE-ACN (3:1) with 10mM of DHLc, 100mM of vinyl ester, 20mg of N435 and 2 molecular sieves 5 angstrom. Error margin = +/- 5%

The scheme for the MAT-MT-MTAN bioalkylation cascade. MAT = Methionine adenosyltransferase, MT = Methyltransferase, MTAN = Methylthioadenosine nucleosidase, SAH = S-adenosylhomocysteine.

The SAM analogue molecular structure. Methyltransferases catalyse the transfer of the R-group (highlighted red) to various substrates, with R representing various alkyl groups.

Process of the sequence retrieving, heterologous expression and characterization of the BVMO originated from Halopolyspora algeriensis.

StEH - epoxide hydrolase from Solanum tuberosum Aldo(M) - V133M/G236V/V250I/G399K mutant of alditol oxidase from Streptomyces coelicolor EcALDH - phenylacetaldehyde dehydrogenase from Escherichia coli

Cry3Aa -formate dehydrogenase (FDH) that is coupled with another enzyme (leucine dehydrogenase or alcohol dehydrogenase) to continuously regenerate NADH cofactors by oxidation of formic acid into CO2 gas, which is then utilized by the second enzyme.

Arginase and ornithine decarboxylase was directly co-immobilized by Cry1Ab protein in vivo. The active particles could be used to promote the conversion of L-arginine to putrescine and its subsequent conversion to nylon 4,6 was also demonstrated.

The biosynthetic route to podophyllotoxin in mayapple, highlighting the penultimate step that is catalysed by DPS

NR: nitrogen recovery. OSB: one-stage batch. B1: sequential batch 1. B2: sequential batch 2.

TAL, tyrosine ammonia lyase

E, enzyme; P, product; S, substrate.

Protein engineering approach towards a highly improved BVMO variant.

pH regimes R1: initial pH 8.0 controlled. R2: initial pH 8.0 uncontrolled. R3: initial pH 6.5 (native) uncontrolled.

pH regimes: R1: initial pH 8.0 controlled. R2: initial pH 8.0 uncontrolled. R3: initial pH 6.5 (native) uncontrolled.

Percentage of eliminated D-glucose in different cycles with P. pastoris immobilized in calcium alginate beads, using a mixture of D-galactose and D-glucose (50 mg/mL of each sugar) as substrate.

Continuous packed bed reactor of L-AI US100 immobilized on Sepabeads EC-EA at 50 °C during 9 days of operation. The flow of the feeding solution (90 g/L galactose, 0.5 mM MnSO4 in 0.1 M MOPS buffer pH 7.5) was maintained at 0.03 mL/min.

DES (deep eutectic solvents)

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BcGT1 catalyzes the transfer of glucosyl residue (red balls) from UDP-glucose to 15-hydroxy cinmethylin (15HCM), releasing 15HCM-β-D-glucoside, and to 15HCM-β-D-glucoside, forming 15HCM β-D-glucosyl-β-D-glucoside (disaccharides).

A biochemical study of KtzT including expression optimization, assay development, activity determinants and structural probing.

Mutation Leu445Ala changes the substrate preference of the enzyme from ω-6 to ω-3 PUFAs and changes the dioxygenation regioselectivity from ω-5 to ω-2 carbon atom on ω-3 PUFAs.

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A. The TK 3M mutant shows a higher flexibility in the active center. B. Dynamics correlation analysis to identify the long=range mutation sites. C. The mutants designed improved the stability of TK 3M without compromise of activity. D. Long-range mutants

A, The C-terminal residue of CgBDH converges at the center of tetramer. B, N258C mutant reconstructed an interaction network involved with active sites. C, Characteristics of wild-type and mutant CgBDH

A – Hydrolysis or transglycosylation catalyzed by rutinosidase using the natural substrate rutin; B – rutinose acting upon a non-natural substrate – 6”-acylated (feruloyl) quercetin 3-O-β-glucopyranoside, demonstrating its broad substrate specificity.

(A): path hypothesis for illustrating the diastereoselectivity of LTA; Dark arrows indicate the entry direction of substrate. Define virtual plane consists of atoms including Cα, HR of PLP-Gly (quinonoid intermediate) and Nɛ, H of catalytic base His86. (B

(A) Models of CpLTA with the docked ligands PLP-Gly.(B): The evolutionary process of CpLTA in diastereoselectivity. syn: mutant with Lsyn-MTPS preference. anti: mutant with L-anti-MTPS preference. conv: conversion rate.

Visual description of ionic liquid polymer gel materials for enzyme entrapment: Structure, properties, versatility and degradation.

Restrained MD-simulations are performed to explore near-attack conformations for selective C4-hydroxylation and characterize relevant binding modes of the model substrate beta-ionone.

Turnover data are mean +/- s.d. of measurements in triplicates. TON (teal bars) determined by GC-MS and enantiomeric excess (red cross) by chiral GC-MS.

HMT-MT cascade transfers fluoromethyl group to small and macromolecules that are subject to further reaction.

CARsr-A was employed to form the CoA derivatives of the respective acids, which were used by enzyme p300 to functionalize peptide H4(1-20)

(A) Production of 11R,12R,13S-trihydroxy-9Z-octadecenoic acid (11R,12R,13S-TriHOME) from linoleic acid (LA) via 12S,13S-epoxy-11R-hydroxy-9Z-octadecenoic acid (12S,13S-EHOME). (B) Production of 11R,12R,13S-trihydroxy-9Z,15Z-octadecadienoic acid (11R,12R,1

(A) Biotransformation of LA in safflower oil hydrolyzate obtained without absorbent resin into 11R,12R,13S-TriHOME. (B) Biotransformation of LA in safflower oil hydrolyzate obtained from absorbent resin treatment into 11R,12R,13S-TriHOME.

Reduction of cyclohexanone (CHO) and acetophenone (ACP) catalyzed by horse live ADH (HLADH) and ADH-A in various DES-water mixtures. HBA: hydrogen bond acceptor, HBD: hydrogen bond donor.

Structural unit of hyaluronan polymer consisted of N-acetyl-D-glucosamine and D-glucuronic acid. Glycosydic linkage types between sugar units are indicated.

Alignment score is 60, 45% sequence identity. we selected one from each cluster for the characterization of formaldehyde-condensing activity.

Aldolase activity was detected by the colorimetric method developed by ourselves, relying on monitoring the consumption of formaldehyde.

(A) Representative bioactive thiazoline and thiazole derivatives. (B) Original function of vanillyl alcohol oxidases (C) chemo-enzymatic synthesis of thiazoline using VAO-mediated oxidation.

The table reports the molar conversion yield of total alpha-glucosides and presents the main structural features of the molecules.

Epigallocatechin glucosides are grouped under monoglucosides (EGCG-G1), diglucosides (EGCG-G2), triglucosides (EGCG-G3) and tetra- and polyglucosides (EGCG-4+).

Strictosidine synthase synthesis of (S)-strictosidine by Pictet-Splenger condensation of tryptamine and secologanine

P450-Glo™ luciferin reactions were carried out with 40nM P450 (Membrane: bacterial membrane preparation / VLP: Encapsulated in VLP (P22) by expression with long scaffold protein) and 50 µM luciferin-MultiCYP. Catalysis was supported using a range of oxyge

Measure of the relative concentration of P450 and total soluble protein during a cycle of recyclability assay, P450 sample incubate for 30min at 37°C in presence of 40 µM Cumene Hydroperoxide (to reproduce reactions conditions). Recyclability was done by

Integrated bioprocess for the upcycling of cheese whey permeate into bio-based surfactants (www.biosurfproject.it).

Schema of the degradation reaction of a polyurethane model system by using an IL/enzyme catalyst system

Screening of enzymatic reactions by flow injection analysis mass spectrometry

An neutron omit map of the involved amino acid residues from two copies of the protein Alcohol dehydrogenase and the water molecules. No density is observed for the central metal ion.

The same spot as in Figure 1 in the crystal as seen by x-rays: Here the metal atom is clearly observed. Figures taken from [1].

Established fatty acid valorisation approaches utilise existing functionalities such as the carboxylate group or pre-existing C=C-double bonds. In this contribution, we add functionality via selective, peroxygenase-catalysed hydroxylation enabling further

Transaminase-triggered SN2-type cyclization to 2-substituted pyrrolidines and piperidines.

This figure shows that our reaction design, inculding reaction mechanism and derivatization of products.

Synthesis of (S)-2-hydroxy-4-arylbut-3-enoic acid (4) through enzymatic cascade reaction.

(A) Generalized scheme for promiscuous intramolecular C-C bond formations using a suitable CvFAP mutant. (B) Photodecarboxylation in an in-house built photoreactor.

Bacteria secrete diffusible molecules during growth. When the concentration reaches a threshold, bacteria synchronize the expression of genes involved in virulence and biofilm. Enzymes able to degrade signal molecules disrupt QS and keep bacteria harmless

(A) Tridimensional structure of SsoPox (dark blue), in complex with an AHL (cyan). The mobile loop (loop 8) is highlighted in green. (B) General structure of QS AHL (HSL, oxo-HSL and OH-HSL). Lactonases degrade AHL by opening lactone rings.

Enzymatic activity of recombinant PhHas-like purified (left). In vivo production of the new polysaccharide (right).

The formation of γ-lactone and the release of resorufin dye are afforded rapidly after the ADH mediated chemoselective reduction of ketone group of levulinate.

ShABS accepts a wide range of carboxylic acids and amines and displayed superior enantioselectivity to different chiral substrates. It also catalyses the synthesis of the pharmaceutical amides ilepcimide and trimethobenzamide.

Crystal structures of ShABS showed a dramatic conformational change of the enzyme which might associate with the different reaction stages. Acid and amine substrate pockets are coloured white and orange, respectively.

Figure 1. Combination of gold species and enzyme for the development of chemoenzymatic cascades

Selective issues and low product concentration in the biocatalytic oxyfunctionalisation of toluene (derivates) addressed by the combined substrate- and reaction engineering approach.

Substrate scope is shown along with product concentration and selectivity. All reactions were conducted under neat condition using immobilised peroxygenase (imm-UPO).

Schematic overview of the semi-automated discovery pipeline developed

The red dashed arrows indicate photosynthetic electron flow. Fd, ferredoxin; P450, cytochrome P450 monooxygenase, S-H, substrate; P-OH, product

Light and dark, reactions in the presence and absence of light, respectively. -Fd and -P450 control reactions without ferredoxin or P450, respectively

Iterative synthesis of peptidoglycan oligosaccharides using a HEWL-glycosynthase/galactosidase tandem from precursors obtained by metabolically engineered bacteria

Proton NMR spectra of DedD protein in presence of peptidoglycan oligosaccharides containing a free lactoyl group or its methyl ester form

Scheme of biocatalytic esterification of FFAs with panthenol and set-ups for scaling-up. Bottom left: picture of panthenyl monoesters with different alkyl-chain lengths

Recovery and chemo-enzymatic transformations of CBD from hemp distilation waste

Semi-logarithmic plot of the enzyme activity on 3-bromo-4-hydroxybenzyl alcohol of indicated EUGOs against the added volume of amino acids in position 166, 391 and 427 (RjEUGO numbering). Enzyme activity increases with the sterical demand of these residue

Enzyme activity on 3-bromo-4-hydroxybenzyl alcohol by natural enzymes in comparison with GcEUGO V166D. The variant was found to be most active in the mutagenesis studies.

Scheme showing PET breakdown into monomers: bishydroxyethyl terephthalate (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), terephthalic acid (TPA) and ethylene glycol (EG).

SEM imagery showing PET powder prior to enzymatic breakdown.

Green Metric Parameters, equations, and definitions. (1,4,5)

A. Radial pentagon in the optimization analysis of substrates molar ratios. B. Assessment of the environmental impact of different set-ups.

SAM analogs used as electrophiles in MTs catalyzed alkylation cascades.

a. Docking of 3-Cl-L-tyrosine with WT-CnTYR: the substrate can fit into the active sites of WT-CnTYR but not in a productive orientation. b. Docking of 3-Cl-L-tyrosine with CnTYR-N201S: the substrate fits well into the active sites of CnTYR-N201S. The fun

A. General workflow for the chemoenzymatic strategy B. Synthesized structurally diverse enantiopure cyclohexanes

A. General workflow for protein engineering B. Substrate scope of MaDA1_M3-catalyzed intermolecular Diels-Alder reactions

The push and pull rational design strategy mechanism of transketolase catalysing a Novel Reaction for Pharmaceutical

Ancestral sequence reconstruction is thereby utilized to identify peripheral and active site mutations in BDH ancestors leading to changes in enantioselectivity.

Figure 1. (S)-Bisoprolol (left) and (S)-betaxolol (right).

Examples of the substrate promiscuity of HSDHs.

Crystal structure of PC-MjMAT. A) The homodimeric structure (blue, dark blue) forms two active sites buried behind a "gating loop" (green) (PDB: 7P84). B) The active site with AdoONB. C) The active site with AdoDMNB (PDB: 7P8M).

A) methylation in nature and application for site-specific B) alkylation, C) photocaging and D) (hetero-) benzylation.

Proposed reaction of the oxidation of indole to indigo by UPOs [3].

Filament formation in the AORAa complex - modelled nanowire complex of composition Aor(AB)5C;

The cascade reactions were catalyzed by a phospholipase A2 (PLA2) and a fatty acid double bond hydratase from S. maltophilia (SmOhyA2).

The biotransformation of soy lecithin (1) into lysolecithin (2) and hydroxy fatty acids (5 and 6) was carried out by the cascade reactions by PLA2 and SmOhyA2.

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The symbols indicate the concentration of oleic acid (closed circle), 1,9-nonanediol (closed down triangle), 9-ANA (closed diamond), 9-amino-1-nonanol (opened down triangle), and 1,9-diaminononane (closed triangle).

Erythrulose (4) can be made from formaldehyde (1) without dihydroxyacetone (3) by combination of EcGCL and VvSucA.

The symbols reflect the change in concentration of formaldehyde (▼), glycolaldehyde (●), dihydroxyacetone (◆) and erythrulose (■) over time.

Chlorite dismutase converts chlorite to molecular oxygen and chloride. Novel reaction intermediates were identified using microsecond timescale rapid kinetics techniques.

Graphical representation of how the solid biocatalytic system that includes both alcohol and glucose dehydrogenases, is able to reduce HMF into BHMF at the same time as it is recycling the available cofactor (NADH).

gene cluster and biosynthetic pathway of pyrrocidine in S. zeae. Structures of the hirsutellone B and xenoacremone C.

Schematic diagram showing the classical side chain oxygenation of styrene biodegradation in gray and the combined conversion of Styrene to PA by SOI in yellow (this study)

Specific activity of the respective NADP+ accepting FDH variants with formamide (F), N-methylformamide (MF) and N,N-dimethylformamide (DMF) as the sole electrone donor at concentrations ranging from 10-40% (v/v).

Scheme of the reductive amination of cyclohexanone catalyzed by AspRedAm coupled with a FDH mutant utilizing N-methylformamide for NADPH regeneration. N-methylformamide serves as the sole electrone and methylamine donor.

The biosynthesis of tryptamine analogues from serine and indole analogues goes through 3 enzymatic steps: an E. coli tryptophanase (EctnaA) condenses the first two building blocks. The obtained tryptophan (or its derivatives) is the substrate for a decarb

Ring-closing metathesis with artificial metalloenzymes based on halide-substituted Grubbs-Hoveyda catalysts.

Left: Conversion of the chiral substrate styrene oxide by StyI determined by GC-FID. StyI shows a strong enantiomeric preference for (S)-styrene oxide, whereas the (R)-epoxide is consumed afterwards. Right: Kinetic resolution of racemic styrene oxide.

Development of an efficient biocatalyst for the production of N-hydroxy-cytidine through evolutionary adaption of the hydrolytic enzyme cytidine deaminase. This engineered biocatalyst performs >85 000 turnovers in less than 3 h.

Development of a synthetic route to Nirmatrelvir, featuring a highly enantio selective biocatalytic de-symmetrization and diastereoselective multicomponent reaction as key steps. The route avoids use of transitions metals and peptide coupling reagents and

Eugenol from Clove[6].(Image adapted from Brian Arthur (CC BY-SA 4.0)) Safrole from Makulan[7]. (Image: Forest & Kim Starr (CC BY 3.0)) Estragole from Estragon[8].

Two different concepts, exploiting oxidation bio/chemistry for the synthesis of fragrance and flavor aldehydes.

General synthetic concept

Figure 1: A. TV74 Nrdjm, B. T.maritima Nrdj reducing the three categories of the nucleotides natural canonical(ATP, ADP), natural non-canonical (ITP/IDP), non-natural non-canonical (6-MPTP, ZDP).

An unspecific peroxygenase from Aspergillus brasiliensis highly expressed in Pichia pastoris catalyses aromatic hydroxylation of benzylic compounds.

Enantiodivergent synthesis of isoindolone derivatives

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Comparison of P450 monooxygenase and peroxygenase catalysed hydroxylation of silanes to silanols

General reactivity of TPLs, and their potential industrial targets.

General reactivity of FDHs, and their potential industrial targets.

Reaction scheme for the synthesis of isotopically labelled L-alanine through use of deuterated NAD2H cofactor, generated in situ

A) Overview of indigo and indican production and dyeing processes. B) Kinetics of indican synthesis using 100 mM indoxyl-acetate as substrate. C) DSF comparing TmB of PtUGT1 WT and designed mutants.

LCA midpoints (C = conventional indigo dyeing, E = enzymatic indican dyeing, P = photolytic indican dyeing)

A novel triple mutant of an extremophilic glycosyl hydrolase allowed the stereoselective, efficient, and sustainable synthesis of still elusive thioglycosides.

Enzymatic reductive amination of ketone 2 with allylamine (1) to provide (S,S,S)-3, which can be easily converted to key component (S,S,S)-5.

IR-09 crystal structure and closer look to the active site with ligand and targeted residues for mutagenesis.

One-pot synthesis of ursodeoxycholic acid using dual NAD-dependent HSDHs, NOX and FDH for cofactor regeneration makes this cascade process green and clean.

Graphical abstract of chimeric enzymes generation and their further characterization.

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Chemo-enzymatic synthesis of azelaic and pelargonic acids from soapstock

From soapstock to oleyl ethanolamide

a: Use of PPK2s for eiter biocatalytic synthesis of NTPs or ATP regeneration coupled to another kinase reaction; b: chemical structures of polyP and an NTP.; c: three-dimensional structure of a class II PPK2 with co-crystallised substrate; d: thermodynami

Hydration of oleic acid catalysed by immobilised oleate hydratase.

Reaction scheme of the asymmetric reduction of different beta-ketoesters by the S-selective NADH-dependent TtHBDH or R-selective NADPH dependent LkKRED

Reaction set-up for Self-Sufficient Heterogeneous Biocatalyst packed in Packed Bed Reactors

Application of StspM1 (immobilized) in preparative scale using a recycling system with the halide methyltransferase (HMT) and S-adenosyl homocysteine (SAH) to methylate the substrate diketopiperazine (LL-DKP).

The OxyMiST project is funded by a six-year grant from the Novo Nordisk Foundation, Grant number NNF20OC0059697.

Melting temperatures indicated by peaks

Irreversible reaction of TK towards benzaldehyde and analogs. X=NO2, Br, F, CO2H

Concept of the enzymatic cascade for biosynthesis of non-canonical (deoxy-) ribonucleotides for the creation of the NucLib chemical library.

SEM image of nano-CLEA particles

The microfluidic system with 3 inlets for ATA-CLEAs generation and their in situ immobilization in a membrane microreactor.

An expression library with different combinations of RBSs and promoters is generated. A C-terminal GFP fusion tag allows the screening and analysis of the expression of a gene of interest (GOI) by FACS and subsequent deep sequencing.

Utilisation of sulphur substrates for alkylation. Organic thiols (black box) are used by ScOAHS with L-homocysteine to synthesise the L-methionine analogues (blue box). When this set up is combined with the alkylation cascade enzymes the utilised RnCOMT t

Occurrence of the nitrosuccinate lyase activity of CreD in Streptomyces cremeus (above) and enzymatic strategies for the hydronitration of unsaturated carboxylic acid using CreD (below).

Confocal microscopy images of flurophore-labelled enzymes at 20X (group of particles) and 40X (individual particle).

Kinetic at 24 hours of each spatial distribution systems: Distribution 1 (on the left) and Distribution 2 (on the right). Rxn mix: 10 mM 1,5-Pentanediol; 1 mM NAD+, 0.15 mM FAD; T(P) 100 mM pH 8

Hydroxylation of Trans-cinnamic acid into р-coumaric acid by the P450 enzyme C4H.

With light as only enery source, reduction equivalents and O2 derived from water splitting are utilized by heterologously expressed Bayer-Villiger-moonooxygenase and lactonase to convert cyclohexanone via caprolacton to 6-hydroxyhexanoic acid.